Prof. Dr. William Orsi

H-index (August, 2023): 38 (Google Scholar), 32 (Web of Science)

(*corresponding author)

Helmbrecht V, Weingart M, Klein F, Braun D, Orsi W* (2023) White and green rust chimneys accumulate RNA in a ferruginous chemical garden. Geobiology 21: 758-769.

Weingart M, Chen S, Donat C, Helmbrecht V, Orsi W, Braun D, Alim K (2023) Alkaline vents recreated in two dimensions to study pH gradients, precipitation morphology, and molecular accumulation. Science Advances 9: eadi1884.

Coskun OK, Gomez-Saez GV, Beren M, Ozcan D, Hosgormez H, Einsiedl F, Orsi W* (2023) Carbon metabolism and biogeography of candidate phylum “Candidatus Bipolaricaulota” in geothermal environments of Biga Peninsula, Turkey. Frontiers in Extreme Microbiology 14: 1063139.

Mincer TJ, Bos ER, Zettler ER, Zhao S, Asbun AA, Orsi W, GuzzettaVS, Amaral-Zettler LA (2023) Sargasso Sea Vibrio bacteria: Underexplored potential pathovars in a perturbed habitat. Water Research 242: 120033.

Orsi W* (2023) A rapid method for measuring ATP+ADP+AMP in marine sediment. Environmental Microbiology 2: 1549-1558.

Orsi W and Inagaki F (2023) Decoding geobiological evolution from microbiomes. Science Advances 9: eadg5448.

Orsi W* (2022) Quantitative microbial ecology: future challenges and opportunities. Environmental Microbiology 25: 91-96.

Vuillemin A, Coskun OK, Orsi W (2022) Microbial activities and selection from surface ocean to subseafloor on the Namibian continental shelf. Applied and Environmental Microbiology 10: e0021622.

Vargas S, Leiva L, Eitel M, Curdt F, Rohde S, Arnold C, Nickel M, Schupp P, Orsi W, Adamska M, Wörheide G (2022) Body-plan reorganization in a sponge correlates with microbiome change. Molecular Biology and Evolution 40: msad138.

Michaelis T, Wunderlich A, Coskun OK, Orsi W, Baumann T, Einsiedl F (2022) High resolution vertical biogeochemical profiles in the hyporheic zone reveal insights into microbial methane cycling. Biogeosciences 19: 4551-4569.

Orsi W*, Vuillemin, Coskun OK, Rodriguez P, Oertel Y, Niggemann J, Mohrholz V, Gomes-Saez G (2022) Carbon assimilating fungi from surface ocean to subseafloor revealed by coupled phylogenetic and stable isotope analysis. The ISME Journal 16: 1245-126

Coskun OK, Vuillemin A, Schubotz F, Klein F, Sichel SE, Eisenreich W, Orsi W* (2022) Quantifying the effects of hydrogen on carbon assimilation in a seafloor microbial community associated with ultramafic rocks. The ISME Journal 16: 257-271.

Orsi W*, Magritsch T, Vargas S, Coskun OK, Vuillemin A, Höhna S, Wörheide G, D’Hondt S, Shapiro J, Carini P (2021) Genome evolution in bacteria isolated from million-year-old subseafloor sediment. mBio 12: e01150-21.

Garber AI, Ramirez GA, McAllister SM, Orsi W, D’Hondt S (2021) Cryptic metabolisms in anoxic subseafloor sediment. Environmental Microbiology Reports 13: 696-701.

Puzenat V, Escartin J, Martel JE, Barreyre T, Le Moine S, Paraskevi N, Gracias N, Allemand P, Antoniou V, Coskun O, Garcia R, Grandjean P, Jørgensen SL, Magi L, Mandalakis M, Orsi W, Polymenakou P, Schouw A, Vallicrosa G, Vlasopoulos O. (2021) Shallow-water hydrothermalism at Milos (Greece): Nature, distribution, heat fluxes and impact on ecosystems. Marine Geology 438; 106521.

E Capo, C Giguet-Covex, A Rouillard, K Nota, P D Heintzman, A Vuillemin, D Ariztegui, F Arnaud, S Belle, S Bertilsson, C Bigler, R Bindler, AG Brown, CL Clarke, SE Crump, D Debroas, G Englund, GF Ficetola, RE Garner, J Gauthier, I Gregory-Eaves, L Heinecke, U Herzschuh, A Ibrahim, V Kisand, KH Kjær, Y Lammers, J Littlefair, E Messager, ME Monchamp, F Olajos, W Orsi, MW Pedersen, DP Rijal, J Rydberg, T Spanbauer, KR Stoof-Leichsenring, P Taberlet, L Talas, C Thomas, DA Walsh, Y Wang, E Willerslev, A van Woerkom, H H Zimmermann, MJL Coolen, LS Epp, I Domaizon, IG Alsos, L Parducci (2021) Lake sedimentary DNA research on past terrestrial and aquatic biodiversity: Overview and recommendations. Quarternary 4: 6.

Einsiedl F, Wunderlich A, Sebilo M, Coskun ÖK, Orsi W, Mayer B (2020) Biogeochemical evidence of anaerobic methane oxidation and anaerobic ammonium oxidation in a stratified lake using stable isotopes. Biogeosciences (accepted) doi.org/10.5194/bg-2020-18

Orsi W*, Morard R, Vuillemin A, Eitel M, Wörheide G, Milucka J, Kucera M. (2020) Anaerobic metabolism of Foraminifera thriving below the seafloor. The ISME Journal 14: 2580-2594.

Orsi W*, Schink B, Buckel W, Martin WF (2020) Physiological limits to life in anoxic subseafloor sediment. FEMS Microbiology Reviews 2: 219-231.

Vuillemin A, Vargas S, Coskun OK, Pockalny R, Murray RW, Smith DC, D’Hondt S, Orsi W (2020) Atribacteria reproducing over millions of years in the Atlantic abyssal subseafloor. mBio 11:e01937-20.

LaRowe DE, Arndt S, Bradley JA, Estes ER, Hoarfrost A, Lang SQ, Lloyd KG, Mahmoudi N, Orsi W, Shah Walter SR, Steen AD, Zhao R (2020) The fate of organic carbon in marine sedements – New insights from recent data and analysis. Earth-Science Reviews 204: 103146.

Girard EB, Kaliwoda M, Schmahl WW, Wörheide G, Orsi W* (2020) Biodegradation of textile waste by marine bacterial communities enhanced by light. Environmental Microbiology Reports 12: 406-418.

Orsi W*, Vuillemin A, Rodriguez P, Coskun O, Gomez G, Mohrholz V, Lavik G, Ferdelman T (2020) Metabolic activity analyses demonstrate that Lokiarchaeon exhibits homoacetogenesis in sulfidic marine sediments. Nature Microbiology 5: 248–255.

Ortega-Arbulu AS, Pichler M, Vuillemin A, Orsi W* (2018) Effects of organic matter and low oxygen on the mycobenthos in a coastal lagoon. Environmental Microbiology doi: 10.1111/1462-2920.14469.

Coskun OK, Pichler M, Vargas S, Gilder S, Orsi W* (2018) Linking uncultivated microbial populations with benthic carbon turnover using quantitative stable isotope probing. Applied and Environmental Microbiology 84(18): e01083-18.

Vuillemin A, Wankel SD, Coskun OK, Magritsch T, Vargas S, Estes ER, Spivack AJ, Smith DC, Pockalny R, Murray RW, D’Hondt S, Orsi W* (2019) Archaea dominate oxic subseafloor communities over multimillion-year timescales. Science Advances 5:eaaw4108.

Coskun O, Özen V, Wankel S, Orsi W* (2019) Quantifying population specific growth in benthic bacterial communities under low oxygen using H218O. The ISME Journal 13, 1546-1559.

Orsi W* (2018) Ecology and evolution of seafloor and subseafloor microbial communities. Nature Reviews Microbiology 16, 671-683.

Ortega-Arbulu AS, Pichler M, Vuillemin A, Orsi W* (2018) Effects of organic matter and low oxygen on the mycobenthos in a coastal lagoon. Environmental Microbiology doi: 10.1111/1462-2920.14469.

Coskun OK, Pichler M, Vargas S, Gilder S, Orsi W* (2018) Linking uncultivated microbial populations with benthic carbon turnover using quantitative stable isotope probing. Applied and Environmental Microbiology 84(18): e01083-18.

Pichler M, Coskun O, Ortega AS, Conci N, Wörheide G, Vargas S, Orsi W* (2018) A 16S rRNA gene sequencing and analysis protocol for the Illumina MiniSeq platform. MicrobiologyOpen e00611.

Orsi W*, Richards TA, Francis WR (2018) Predicted microbial secretomes and their target substrates in marine sediments. Nature Microbiology 3: 32-37.

Vuillemin A, Horn F, Friese A, Winkel M, Alawi M, Wagner D, Henny C, Orsi W, Crowe SA, Kallmeyer J (2018) Metabolic potential of microbial communities from ferruginous sediments. Environmental Microbiology doi: 10.1111/1462-2920.14343.

Vuillemin A, Ariztegui D, Horn F, Kallmeyer J, Orsi W, the PASADO Science Team (2018) Microbial community composition along a 50,000 year lacustrine sediment sequence. FEMS Microbiology Ecology doi: 10.1093/femsec/fiy029/4880442.

Kose SH, Grice K, Orsi W, Ballal M, Coolen MJL (2018) Metagenomics of pigmented and cholesterol gallstones: the putative role of bacteria. Scientific Reports 8, 11218.

Giosan L, Orsi W, Coolen M, Wuchter C, Dunlea AG, Thirumalai K, Munoz S, Clift PD, Donnelly JP, Galy V, Fuller DQ (2018) Neoglacial climate anomalies and the Harappan Metamorphosis. Climate of the Past doi.org/10.5194/cp-2018-27.

More KD, Orsi W, Galy V, Giosan L, He L, Grice K, Coolen MJL (2018) A 43 kyr record of protist communities and their response to oxygen minimum zone variability in the Northeastern Arabian Sea. Earth and Planetary Science Letters 496, 248-256.

Orsi W*, Wilken S, del Campo J, Heger T, James E, Richards TA, Keeling PJ, Worden AZ, Santoro AE (2018) Identifying protist consumers of photosynthetic picoeukaryotes in the surface ocean using stable isotope probing. Environmental Microbiology 20, 815-827

He K, Gilder SA, Orsi W, Zhao X, Petersen N (2017) Constant flux of spatial niche partitioning through high-resolution sampling of magnetotactic bacteria. Applied and Environmental Microbiology 83(20) e01382-17.

Orsi W*, Coolen MJ, Wuchter C, He L, More KD, Irigoien X, Chust G, Johnson C, Hemingway JD, Lee M, Galy V, Giosan L (2017) Climate oscillations reflected in the microbiome of Arabian Sea sediments. Scientific Reports 7: 6040.

Orsi W, Jørgensen BB, Biddle JF (2016) Transcriptional analysis of sulfate reducing and chemolithoautotrophic sulfur oxidizing bacteria in the deep subseafloor. Environmental Microbiology Reports 8, 452-460.

Orsi W, Smith JM, Liu S, Liu Z, Sakamoto CM, Wilken S, Poirier C, Richards TA, Keeling PJ, Worden AZ, Santoro AE (2016) Diverse, uncultivated bacteria and archaea underlying the cycling of dissolved protein in the ocean. The ISME Journal 10, 2158-2173.

Klein F, Humphris SE, Guo W, Schubotz F, Schwarzenbach EM, Orsi WD (2015) Fluid mixing and the deep biosphere of a fossil Lost City-type hydrothermal system at the Iberia Margin. PNAS 112, 12036-12041.

Orsi W, Richards TA, Santoro AE (2015) Cellular maintenance processes potentially underpin the survival of subseafloor fungi over geological timescales. Estuarine Costal and Shelf Science (10.1016/j.ecss.2015.04.009).

Orsi W, Smith JM, Wilcox HM, Swalwell JE, Carini P, Worden AZ, Santoro AE (2015). Ecophysiology of uncultivated marine euryarchaea is linked to particulate organic matter. The ISME Journal 9: 1747-1763.

Coolen MJL* and Orsi W* (2015) The transcriptional response of microbial communities in thawing Alaskan permafrost soils. Frontiers in Microbiology 6:197. *Co-first authors.

Engelhardt T, Orsi W, Jørgensen BB (2015) Viral activities and life-cycles in deep subseafloor sediment. Environmental Microbiology Reports doi:10.1111/1758-2229.12316.

Santoro AE, Dupont CL, Richter RA, Craig MT, Carini P, McIlvin MR, Yang Y, Orsi W, Moran D, Saito MA (2015). Genomic and proteomic characterization of ‘Candidatus Nitrosopelagicus brevis’: an ammonia-oxidizing archaeon from the open ocean. PNAS 114:1173-1178.

Orcutt BN, LaRowe D, Lloyd K, Mills H, Orsi W, Reese BK, Sauvage J, Huber JA, Amend J. (2014) IODP deep biosphere research workshop report – a synthesis of recent investigations, and discussion of new research questions and drilling targets. Scientific Drilling 2:1-6.

Stoeck T, Filker S, Edgcomb V, Orsi W, Yakimov M, Pachiadaki M, Breiner HW, LaCono V, Stock A. (2014) Living at the limits: Evidence for microbial eukaryotes thriving under pressure in deep anoxic, hypersaline habitats. Advances in Ecology doi:10.1155/2014/532687

Orsi W, Edgcomb V, Christman G, Biddle J. (2013). Gene expression in the deep biosphere. Nature 499: 205-208.

Coolen MJL, Orsi W, Balkema C, Quince C, Harris K, Sylva S, Filipova-Marinova M, Giosan L. (2013). Evolution of the plankton paleome in the Black Sea from the Deglacial to Anthropocene. PNAS 110(21): 8609-14.

Orsi W, Biddle J, Edgcomb, V. (2013). Deep sequencing of subseafloor eukaryotic rRNA reveals active Fungi across multiple subseafloor provinces. PLoS ONE 8(2): e56335.

Burgaud G, Woehlke S, Redou V, Orsi W, Beaudoin D, Barbier G, Biddle J, Edgcomb V. (2013). Deciphering presence and activity of fungal communities in marine sediments using a model estuarine system. Aquatic Microbial Ecology 70: 45-62.

Stock A, Edgcomb V, Orsi W, Filker S, Breiner HW, Yakimov M, Stoeck T. (2013). Evidence for isolated evolution of deep-sea ciliate communities through geological separation and environmental selection. BMC Microbiology 13:150.

Edgcomb V, Bernhard J, Summons R, Orsi W, Beaudoin D, Visscher P. (2013). Active eukaryotes in microbialites from Highborne Cay, Bahamas, and Hamelin Pool (Shark Bay), Australia. The ISME Journal. DOI:10.1038/ismej.2013.130.

Edgcomb V, Orsi W, Biddle J. (2013) Fungi in the marine subsurface; In: Kallmeyer, J. and Wagner, D. (eds.), Microbial life of the Deep Biosphere. (in press) De Gruyter, Berlin.

Orsi W, Song Y, Hallam S, Edgcomb V. (2012). Effect of oxygen minimum zone formation on communities of marine protists. The ISME Journal 6: 1586-1601.

Orsi W, Edgcomb V, Faria J, Foissner W, Fowle W, Hohmann T, Suarez S, Taylor C, Taylor GT, Vdacny P, Epstein S. (2012). Class Cariacotrichea, a novel ciliate taxon from the anoxic Cariaco Basin, Venezuela; International Journal of Systematic and Evolutionary Microbiology 62: 1425-1433.

Orsi W, Charvet S, Vd’ačný P, Bernhard JM, Edgcomb V. (2012). Prevalence of partnerships between bacteria and ciliates in oxygen-depleted marine water columns. Frontiers in Extreme Microbiology 3: 341.

Vdacny P, Bourland W, Orsi W, Epstein S, Foissner W. (2012). Genealogical analyses of multiple loci of litostomatean ciliates (Protista, Ciliophora, Litostomatea). Molecular Phylogenetics and Evolution 65(2): 397-411.

Filker S, Stock A, Breiner H W, Edgcomb V, Orsi W, Yakimov M, Stoeck T. (2012). Environmental selection of protistan plankton communities in hypersaline anoxic deep-sea basins, Eastern Mediterranean Sea. MicrobiologyOpen 2(1): 54-63.

Orsi W and Edgcomb V. (2012). Microbial Eukaryotes in Oxygen Minimum Zones; In: Seckbach, J., Oren, A., and Stan-Lotter, H. (eds.), Polyextremophiles – Organisms living under multiple stress; Springer, Dordrecht.

Edgcomb V and Orsi W. (2012). Microbial Eukaryotes in Hypersaline Anoxic Deep Sea Basins; In: Seckbach, J., Oren, A., and Stan-Lotter, H. (eds.), Polyextremophiles – Organisms living under multiple stress; Springer, Dordrecht.

Orsi W, Edgcomb V, Jeon S O, Leslin C, Bunge J, Taylor G T, Varela R, Epstein S. (2011). Protistan microbial observatory in the Cariaco Basin, Caribbean. Part II. Habitat specialization. The ISME Journal 5:1344-1356.

Edgcomb V, Orsi W, Bunge J, Jeon S O, Leslin C, Christen R, Holder M, Taylor G T, Suarez P, Varela R, Epstein S. (2011). Protistan microbial observatory in the Cariaco Basin, Caribbean. Part I. Pyrosequencing vs. Sanger insights into species richness. The ISME Journal 5: 1357-1373.

Edgcomb V, Orsi W, Taylor G T, Vd’ačný P, Taylor C, Suarez P, Epstein S. (2011). Commentary: Accessing marine protists from the anoxic Cariaco Basin. The ISME Journal 5: 1237-1241.

Edgcomb V, Orsi W, Breiner HW, Stock A, Filkner S, Yakimov M, Stoeck T. (2011). Eastern Mediterranean deep hypersaline anoxic lakes: novel active kinetoplastids associated with halocline habitats. Deep–Sea Research I 58 (10): 1040-1048.

Vd’ačný P, Bourland W, Orsi W, Epstein S, Foissner W. (2011). Phylogeny and classification of the Litostomatea (Protista, Ciliophora), with an emphasis on free-living taxa and the 18S rRNA gene. Molecular Phylogenetics and Evolution 59 (2): 510-522.

Vd’ačný P, Orsi W, Bourland W, Satoshi S, Epstein S, Foissner W. (2011). Morphological and molecular phylogeny of Dileptids: resolution at the base of the class Litostomatea (Ciliophora, Intramacronucleata). European Journal of Protistology 47 (4): 295-313.

Vd’ačný P, Orsi W, Foissner W. (2010). Molecular and morphological evidence for a sister group relationship of the classes Armophorea and Litostomatea (Ciliophora, Intramacronucleata, Lamellicorticata infraphyl. nov.), with an account on basal haptorid litostomateans. European Journal of Protistology 46 (4): 298-309.

Edgcomb V, Orsi W, Leslin C, Epstein SS, Bunge J, Jeon S, Yakimov MM, Behnke A, Stoeck T. (2009). Protistan community patterns within the brine and halocline of deep hypersaline anoxic basins in the eastern Mediterranean Sea. Extremophiles 13 (1): 151-167.

Stoeck T, Behnke A, Christen R, Amaral-Zettler L, Rodriguez-Mora MJ, Chistoserdov A, Orsi W, Edgcomb VP. (2009). Massively parallel tag sequencing reveals the complexity of anaerobic marine protistan communities. BMC Biology 7:72.